Mechanisms and Specificity of Phenazine Biosynthesis Protein PhzF
نویسندگان
چکیده
منابع مشابه
Structure and function of the phenazine biosynthesis protein PhzF from Pseudomonas fluorescens 2-79.
Phenazines, including pyocyanin and iodonin, are biologically active compounds that are believed to confer producing organisms with a competitive growth advantage, and also are thought to be virulence factors in certain diseases including cystic fibrosis. The basic, tricyclic phenazine ring system is synthesized in a series of poorly characterized steps by enzymes encoded in a seven-gene cistro...
متن کاملElucidation of Enzymatic Mechanism of Phenazine Biosynthetic Protein PhzF Using QM/MM and MD Simulations
The phenazine biosynthetic pathway is of considerable importance for the pharmaceutical industry. The pathway produces two products: phenazine-1,6-dicarboxylic acid and phenazine-1-carboxylic acid. PhzF is an isomerase that catalyzes trans-2,3-dihydro-3-hydroxyanthranilic acid isomerization and plays an essential role in the phenazine biosynthetic pathway. Although the PhzF crystal structure ha...
متن کاملPhenazine biosynthesis by a pseudomonad.
The acidophilic bacterium Pseudomonas phenazinium (N.C.I.B. 11027), isolated from soil by virtue of its ability to grow on L-threonine as sole source of carbon and nitrogen (Bell et al., 1972), was observed to produce the water-insoluble purple pigment iodinin (phenazine-l,6-diol 5,lO-dioxide) during growth on L-threonine or glycine media (Bell & Turner, 1973). Evidence was obtained that iodini...
متن کاملThe structural biology of phenazine biosynthesis.
The phenazines are a class of over 150 nitrogen-containing aromatic compounds of bacterial and archeal origin. Their redox properties not only explain their activity as broad-specificity antibiotics and virulence factors but also enable them to function as respiratory pigments, thus extending their importance to the primary metabolism of phenazine-producing species. Despite their discovery in t...
متن کاملStructure of the phenazine biosynthesis enzyme PhzG.
PhzG is a flavin-dependent oxidase that is believed to play a role in phenazine antibiotic synthesis in various bacteria, including Pseudomonas. Phenazines are chorismic acid derivatives that provide the producing organisms, including the opportunistic pathogen P. aeruginosa, with a competitive growth advantage. Here, the crystal structures of PhzG from both P. aeruginosa and P. fluorescens sol...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-06278-w